====== 脱水 ======

[[:]]では[[acidification]]の後に行う。
一般的にはもう少し後の工程で行なわれるが、
[[:]]では次に行う[[dewaxing]]で水分が無い方が良い(方法が違うためか?)。

===== 方法 =====

検体は[[soaking dish]]にあるものとする。

(1) 前の処理で使用した薬液を[[decantation]]する。

(2) 容器のフタなどについた薬液をティッシュペーパー等で拭う。

(3) [[dehydration agent]]を0.5mLぐらい加えて常温で5分ぐらい静置する。

(4) [[dehydration agent]]を[[decantation]]する。

(5) [[dehydration agent]]を0.5mLぐらい加えて常温で5分ぐらい静置する。




===== 調査 =====

文献に書かれている情報。
どの文献でもだいたい同じような感じ。

> <wrap :en>
6. Decant bleach or stain and twice rinse specimens in Glacial Acetic Acid* or 95% Ethanol. \\
7. Final dehydration of specimens may be carried out by soaking in Glacial Acetic Acid* or Absolute
Ethanol for a few minutes. Decant dehydration fluid. \\
... \\
* The choice between Glacial Acetic Acid and alcohols, is influenced by economic and safety factors.
Glacial Acetic Acid has the advantage of being a cheap means of neutralising alkalis and vigorously dehydrating material, and provides the acid medium necessary for staining; it has the disadvantages of its unpleasant, breath-catching smell and ability to cause skin burns.
Whilst 95% industrial Ethanol (IMS) is cheap when it can be readily obtained, Absolute (100%) Ethanol is extremely expensive, and both are more pleasant to work with than Acetic Acid.
Ethanol is extremely hygroscopic, however, and many workers prefer 100% iso-propanol, which is not.
Iso-propyl alcohol is, thus, a better choice for dehydration in humid environments, as well being cheaper than Absolute Ethanol.
[(brown2006>>pages:16)] </wrap>




===== 参考文献 =====
~~REFNOTES~~

<wrap small>
[[:refnotes:brown2006]],
</wrap>

===== Backlinks =====
{{backlinks>.}}

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===== TODO =====
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