dehydration
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| 次のリビジョン | 前のリビジョン | ||
| dehydration [2025/02/16 20:14] – 作成 Konajirami-ya | dehydration [2026/04/24 11:38] (現在) – Konajirami-ya | ||
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| ====== 脱水 ====== | ====== 脱水 ====== | ||
| - | <WRAP center round todo 60%> | + | [[: |
| - | 工事中 | + | 一般的にはもう少し後の工程で行なわれるが、 |
| - | </WRAP> | + | [[: |
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| + | ===== 方法 ===== | ||
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| + | 検体は[[soaking dish]]にあるものとする。 | ||
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| + | (1) 前の処理で使用した薬液を[[decantation]]する。 | ||
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| + | (2) 容器のフタなどについた薬液をティッシュペーパー等で拭う。 | ||
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| + | (3) [[dehydration agent]]を0.5mLぐらい加えて常温で5分ぐらい静置する。 | ||
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| + | (4) [[dehydration agent]]を[[decantation]]する。 | ||
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| + | (5) [[dehydration agent]]を0.5mLぐらい加えて常温で5分ぐらい静置する。 | ||
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| + | ===== 調査 ===== | ||
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| + | 文献に書かれている情報。 | ||
| + | どの文献でもだいたい同じような感じ。 | ||
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| + | > <wrap :en> | ||
| + | 6. Decant bleach or stain and twice rinse specimens in Glacial Acetic Acid* or 95% Ethanol. \\ | ||
| + | 7. Final dehydration of specimens may be carried out by soaking in Glacial Acetic Acid* or Absolute | ||
| + | Ethanol for a few minutes. Decant dehydration fluid. \\ | ||
| + | ... \\ | ||
| + | * The choice between Glacial Acetic Acid and alcohols, is influenced by economic and safety factors. | ||
| + | Glacial Acetic Acid has the advantage of being a cheap means of neutralising alkalis and vigorously dehydrating material, and provides the acid medium necessary for staining; it has the disadvantages of its unpleasant, breath-catching smell and ability to cause skin burns. | ||
| + | Whilst 95% industrial Ethanol (IMS) is cheap when it can be readily obtained, Absolute (100%) Ethanol is extremely expensive, and both are more pleasant to work with than Acetic Acid. | ||
| + | Ethanol is extremely hygroscopic, | ||
| + | Iso-propyl alcohol is, thus, a better choice for dehydration in humid environments, | ||
| + | [(brown2006>> | ||
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| - | <WRAP hide>< | ||
| ===== 参考文献 ===== | ===== 参考文献 ===== | ||
| ~~REFNOTES~~ | ~~REFNOTES~~ | ||
| - | </nowiki></WRAP> | + | |
| + | <wrap small> | ||
| + | [[: | ||
| + | </wrap> | ||
| ===== Backlinks ===== | ===== Backlinks ===== | ||
dehydration.1739704495.txt.gz · 最終更新: 2025/02/16 20:14 by Konajirami-ya
