検体は浸漬皿にあるものとする。
(1) 前の処理で使用した薬液を️排液する。
(2) 容器のフタなどについた薬液をティッシュペーパー等で拭う。
(3) 脱水剤を0.5mLぐらい加えて常温で5分ぐらい静置する。
(5) 脱水剤を0.5mLぐらい加えて常温で5分ぐらい静置する。
文献に書かれている情報。 どの文献でもだいたい同じような感じ。
6. Decant bleach or stain and twice rinse specimens in Glacial Acetic Acid* or 95% Ethanol.
7. Final dehydration of specimens may be carried out by soaking in Glacial Acetic Acid* or Absolute Ethanol for a few minutes. Decant dehydration fluid.
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* The choice between Glacial Acetic Acid and alcohols, is influenced by economic and safety factors. Glacial Acetic Acid has the advantage of being a cheap means of neutralising alkalis and vigorously dehydrating material, and provides the acid medium necessary for staining; it has the disadvantages of its unpleasant, breath-catching smell and ability to cause skin burns. Whilst 95% industrial Ethanol (IMS) is cheap when it can be readily obtained, Absolute (100%) Ethanol is extremely expensive, and both are more pleasant to work with than Acetic Acid. Ethanol is extremely hygroscopic, however, and many workers prefer 100% iso-propanol, which is not. Iso-propyl alcohol is, thus, a better choice for dehydration in humid environments, as well being cheaper than Absolute Ethanol. (P. Brown, E. Boise, 2006, pp.16)
Brown, P. & Boise, E. (2006) Procedures for preparation and conservation …,
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